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OBJECTIVE： To study the effects of exogenous methyl jasmonate on the contents of 4 kinds of secoiridoid substance （gentiopicrin， swertiamain， sweroside and amarogentin） and biochemical indexes in the leaves of Gentiana rigescens， and to provide scientific evidence for the application of methyl jasmonate in standardized planting of G. rigescens. METHODS： 2， 4， 6， 8， 10 d after spraying 10， 50， 100， 200 mg/L methyl jasmonate （spraying amount was 500 mL） for G. rigescens strain， the leaves of medicinal material were collected as sample， and the other leaves without spraying methyl jasmonate were collected as control. HPLC method was used to determine the contents of 4 kinds of secoiridoid substance in the leaves of G. rigescens after spraying 4 kinds of concentrations of methyl jasmonate for 10 d. The concentration of methyl jasmonate was optimized （the content of effective component was the highest）. HPLC method was adopted to determine the contents of 4 kinds of secoiridoid substance in the leaves of G. rigescens after spraying the optimal concentration of methyl jasmonate for different time. The levels of relevant biochemical indexes （SOD， POD， CAT， MDA） were determined. RESULTS： 10 d after spraying 10， 50， 100， 200 mg/L methyl jasmonate for G. rigescens strains， the contents of 4 kinds of secoiridoid substance in the leaves were increased to different extent. Compared with untreated leaves， 100 mg/L methyl jasmonate had the best effect after spraying， and the contents of gentiopicrin， swertiamain and sweroside in treated leaves were 1.88， 2.36 and 1.87 times of those in untreated leaves， respectively （P＜0.05）. 2， 4， 6， 8， 10 d after spraying 100 mg/L methyl jasmonate， the contents of 4 kinds of secoiridoid substance in treated leaves were higher than untreated leaves at corresponding stage； the content of secoiridoid had significant difference after spraying for 4 d （P＜0.05）. The contents of active components in general were relatively high after spraying for 6 d， and the contents of gentiopicrin， swertiamain， sweroside and amarogentin in treated leaves were 1.88， 1.88， 1.47， 1.82 times of those in untreated leaves， respectively （P＜0.05）. The levels of relevant biochemical indexes in treated leaves were increased significantly since 4 d of spraying， compared with untreated leaves （P＜0.05）. CONCLUSIONS： After spraying 100 mg/L methyl jasmonate for 6 d， the contents increase of 4 kinds of secoiridoid substance in the leaves of G. rigescens are most obvious， which may be associated with improving the levels of related biochemical indexes.
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Objective:To analyze and determine heavy metal content in Paridis Rhizoma from different genus and localities,in order to provide a reference for selecting cultivation areas and establishing the quality standard of Paridis Rhizoma of heavy metals content. Method:Microwave digestion method combined with inductively coupled plasma atomic emission spectrometry method (ICP-AES) method were applied to determine the contents of 6 heavy metals,i.e. As,Cu,Hg,Cd,Pb and Cr in 39 samples of Paridis Rhizoma of different genus and localities in Yunnan Province. Cluster analysis,statistical analysis and principal component analysis (PCA) were used to compare the differences of heavy metals contents in different localities and species. Result:The contents of six heavy metals in Paridis Rhizoma met the ISO international standard of Heavy Metal Limit of Traditional Chinese Medicine-traditional Chinese Medicinal Materials. Under the limited value standard of Green Trade Standards of Importing Medicinal Plants and Preparations,the over-standard rate of heavy metal As was 15.4%,the excess rate of Cd was 5.1%,and the excess rate of Pb was 2.6%. The contents of Cu and Hg conformed to relevant requirements. Cluster analysis,statistical analysis and principal component analysis showed that for the same variety,differences in producing places had significant effects on heavy metal content,while differences in species had little effects. Conclusion:The results of this study indicated that the heavy metal content of Paris planted in and around Dali basically conformed to relevant standards. The differences of heavy metal content in Paris were mainly regional differences,which provided a theoretical basis for standardizing the cultivation of medicinal materials and formulating the limit standards of heavy metals for Paridis Rhizoma.
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Objective To explore the application of left colon artery and retention of anal canal in rectal cancer anterior resection .Methods 134 patients with rectal resection were divided into two groups by random number table.The observation group (n=67) received anterior resection of the left colonic artery and retained anorectal anterior resection.The control group(n=67) received subconjunctival artery root ligation ,non -retained anorectal anterior resection of rectal cancer .The number of lymph node dissection and the incidence of postoperative anastomotic leakage were compared between the two groups .The rate of reoperation was compared between the two groups .Results The number of lymph node dissection in the observation group and control group was (4.2 ±1.3),(4.4 ±1.2),the difference was not statistically significant (P=0.450).The incidence rate of anastomotic leakage was 2.99％ in the observation group,which was 11.94％in the control group,the difference was statistically significant (χ2 =3.890,P=0.049).Conclusion In the case of no difference in preoperative and postoperative perioperative management ,the left colonic artery and the anal canal were retained in the rectal cancer without affecting the lymph node dissection in No.253 group,but it could provide sufficient blood for the anastomosis ,to reduce the risk factor of the occurrence of anastomotic leakage .
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<p><b>OBJECTIVE</b>To establish a high performance liquid chromatography method for the determination of tetrahydropulmatine content in treatment of winter's diseases in summer path.</p><p><b>METHOD</b>The analysis was performed on the Hypersil ODS2 column (4.6 mm x 250 mm, 5 microm) with methanol and 0.1% phosphoric acid solution (adjusted pH to 6.28 with triethylamine) as mobile phase at 63:37. The flow rate was 1.0 mL x min(-1) and the UV detection wavelength was 280 nm.</p><p><b>RESULT</b>The linear range of tetrahydropulmatine was obtained from the range of 2.08 - 49.92 mg x L(-1) (r = 0.999 8). The average recovery was 97.85% with RSD 2.49%.</p><p><b>CONCLUSION</b>This method is simple, reliable and can be used for the determination of tetrahydropulmatine in this preparation.</p>
Subject(s)
Chromatography, High Pressure Liquid , Methods , Drugs, Chinese Herbal , Chemistry , Reproducibility of ResultsABSTRACT
OBJECTIVE:To establish the quality standard of Baji zhenyang tablets.METHODS:Panax ginseng,Herba Epimedii and Carthamus tinctorius in the tablets were identified by TLC,and the content of icariin in Herba Epimedii was determined by HPLC.RESULTS:The TLC spots of P.ginseng,Herba Epimedii and C.tinctorius were distinctive and well-separated.The linear range of icariin was 0.080 04～0.640 32 ?g(r=0.999 8).The average recovery was 101.29%(RSD=0.98%,n=6).CONCLUSION:The established standard is applicable for the quality control of Baji zhenyang tablets.
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OBJECTIVE: To compared the similarity between Cordyceps Sinensis and Solid Fermentation of Cordyceps Militaris in chemical components by TLC. METHODS: The similarity in total constituents and different extract fractions were compared between Cordyceps Sinensis and Solid Fermentation of Cordyceps Militaris by TLC. RESULTS: Solid Fermentation of Cordyceps Militaris and Cordyceps Sinensis were basically similar in that their TLC spots occurred at the corresponding place except a slight difference in size. CONCLUSION: Cordyceps Sinensis and Solid Fermentation of Cordyceps Militaris have similar chemical constituents, which remains to be further studied.
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Objective For selecting and developing the excellent Bupleurum chinense to mass-produce seedlings and seeds in high quality.Methods B.chinense was picked from eight different areas,such as Lingchuan and Wanrong county in Shanxi Province,Longxi county in Gansu Province,and Shangluo in Shaanxi Province,etc.Rapid propagation was done.The testa of Zhongchai No.1 was scrapped or seeds were soaked in different phytohormones.The effects on germination rates of seeds were compared.ResultsThe optimum medium for bud propagation was B5supplemented with 1.0 mg/L 6-BA and 0.2 mg/L KT.The optimum medium for root induction of test-tube plantlets was 1/2 MS added with 0.1 mg/L NAA,0.5 mg/L IBA,and 1.0 mg/L DSC.The annual propagation coefficient of B.chinense plants was more than 1?108,and the survival rate of transplantation reached to 94%—97%.The phytohormone has little effect on seed germination of B.chinense,but scraping the testa could increase the germination percentage of seeds to 20% and shorten the germinating time greatly.Conclusion By tissue culture of excellent B.chinense,a great deal of plants and seeds could be produced in short time.By scrapping testa in a certain extent,the germination of seeds could be increased.